Patient ID must be confirmed before drawing blood. Prior to collecting samples, the phlebotomist will ask the patient their name and verify that the answer matches the name on the request.

"Universal Precautions" should be followed in handling all items contaminated with blood or body fluids. Personal protective equipment such as gloves and eye protection should be worn when performing phlebotomy procedures.

All tubes and collection containers will be labeled at the time of collection with the patients first and last name, the patients date of birth or other identifier such as SS#, the date and time of collection and the initials of the phlebotomist collecting the specimen.

Prior to blood collection, verify the patient's diet restrictions, as appropriate.

Select the best possible vein site and cleanse with an alcohol swab. Apply tourniquet for no longer than one minute. Perform the venipuncture using the correct order of draw. Release and remove tourniquet. Ensure the patient's hand is open. Place a gauze pad over the puncture site and remove the needle. Bandage the patients arm. Discard needles, syringes and contaminated collection materials in sharps container and biohazard waste containers.

All tubes containing additives should be inverted five to ten times to mix the contents. Serum specimens (red top/ tiger top) should be inverted two times and allowed to clot in an upright position at room temperature for 20-30 minutes prior to centrifugation. Serum specimens should be centrifuged @ 1500 rpm for 15 minutes. Serum specimens should be refrigerated until tested. Lavender top tubes for hematology testing should be stored at room temperature until tested.

Order of draw:
  • Blood Cultures
  • Royal Blue
  • Plain Red
  • Red-Gray Tiger Top (Serum Separator Tubes)
  • Light Blue
  • Green
  • Lavender
  • Gray